Oxidation of Thiocyanate to Cyanide Catalyzed by Hemoglobin
نویسندگان
چکیده
منابع مشابه
Oxidation of thiocyanate to cyanide catalyzed by hemoglobin.
Thiocyanate ion is oxidized at acid pH by hydrogen peroxide to sulfate and cyanide. The reaction is catalyzed in the erythrocyte by hemoglobin acting as a peroxidase (donor: Hz02 oxidoreductase, EC 1.11.1.7). Kinetic studies show thiocyanate ion fulfdls criteria for a substrate for the peroxidase. The peroxidase activity is enhanced by haptoglobin and inhibited by azide, aminotriazole, fluoride...
متن کاملEnzymatic conversion of cyanide to thiocyanate.
T HE minute amounts of thiocyanate normally present in urine, blood and saliva have stimulated the interest of many workers as to the origin of the cyanide and the pattern of conversion of cyanide to thiocyanate. The problem has toxicologic as well as physiologic importance because of the great toxicity of cyanide. The detoxication of cyanide in the animal body was first demonstrated by S. Lang...
متن کاملLow catalytic turnover of horseradish peroxidase in thiocyanate oxidation. Evidence for concurrent inactivation by cyanide generated through one-electron oxidation of thiocyanate.
The catalytic turnover of horseradish peroxidase (HRP) to oxidize SCN- is a hundredfold lower than that of lactoperoxidase (LPO) at optimum pH. While studying the mechanism, HRP was found to be reversibly inactivated following pseudo-first order kinetics with a second order rate constant of 400 M-1 min-1 when incubated with SCN- and H2O2. The slow rate of SCN- oxidation is increased severalfold...
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A cyanide-laden effluent of the gold mining industry must be treated before it can be released to the aquatic environment. Current treatment methods such as natural degradation, sulfur based, hydrogen peroxide, ozonation, and alkaline chlorination are not adequate for meeting newer treatment standards. A new chemical oxidant that utilizes iron in the +6 oxidation state, ferrate [Fe(VI)] was stu...
متن کاملMilk transfer of cyanide and thiocyanate: Cyanide exposure by lactation in goats.
The present work was aimed at evaluating the effects of maternal exposure to potassium cyanide (KCN) during lactation in goats. Twenty-seven lactating female goats were orally dosed with 0 (control), 1.0, 2.0, or 3.0 mg KCN/kg body weight/day from lactation days 0 to 90. After this period, all male kids and one mother from each group were killed for a pathological study. Cyanide treatment promo...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62450-0